INTRODUCTION. To correct disorders in the male reproductive system organs in the metabolic syndrome, it seems reasonable to use a combined effect of natural and artificial therapeutic physical factors – drinking mineral water and low-intensity electromagnetic interference of ultrahigh frequency – on an experimental model of this disease. These factors have antioxidant, cytoprotective and regenerative effects and can have a wide range of effects on various adaptation and protective regulatory mechanisms. AIM. To reveal the development of metabolic and structural adaptation-protective reactions in rat testes under the combined effect of drinking sulphate mineral water and low-intensity electromagnetic radiation microwave in experimental simulation of metabolic syndrome. MATERIAL AND METHODS. The experiments were performed on 26 nonlinear male rats weighing 180-200g. The metabolic syndrome model was reproduced for 60 days using a high-caloric diet. The rats were divided into 3 groups: 1st (experimental)-rats received drinking sulfate mineral water in combination with microwave electromagnetic radiation against the background of a high-calorie diet; 2nd (control) – rats received only a high-calorie diet; 3rd (intact) – rats without any influences. Research methods: biochemical, lightoptical, morphometric, electron microscopy. Significance of differences was assessed by Student's t-test. RESULTS AND DISCUSSION. It was found that the combinational effect of drinking sulfate mineral water and low-intensity electromagnetic radiation microwave contributed to the enhancement of a number of adaptive and protective reactions in the testes of rats against the background of a high-caloric diet. They were mainly manifested in the activation of antioxidant protection and protein synthesis, improvement of spermatogenic cells differentiation processes and increase in their number, enhancement of cellular and intracellular regeneration. CONCLUSION. The data obtained can be used in the development of new approaches to the methods of adaptation-protective and compensatory-restorative processes enhancement in the organs of the male reproductive system in metabolic syndrome.
metabolic syndrome, mineral water, low-intensity electromagnetic radiation, testis
1. Roytberg G.E. Metabolicheskiy sindrom. Moskva. MED press-inform. 2007: 224 s.
2. Lemieux I., Despers J.P. Metabolic Syndrome: Past, Present and Future. Nutrients. 2020; 12(11): 3501. https://doi.org/10.3390/nu12113501
3. Leisegang K., Sengupta P., Agarval A., Henkel R. Obesity and male infertility: Mechanisms and management. Andrologia. 2021; 53(1): e13617. https://doi.org/10.1111/and.13617
4. Cohen D.J., Giaccagli M.H., Herzfeld J.D., Gonzalez L.N., Cuasnicu P.S. Da Ros V.G. Metabolic syndrome and male fertility disorders: Is there a causal link? Reviews in Endocrine and Metabolic Disorders. 2021; 22(4): 1057-1071. https://doi.org/10.1007/s11154-021-09659-9
5. Martins A.D., Majzoub A., Agawal A. Metabolic syndrome and male fertility. The World Journal of Men’s Health. 2019; 37(2): 113-127. https://doi.org/10.5534/wjmh.180055
6. Lotti F., Marchiani S., Corona G., Maggi M. Metabolic syndrome and reproduction. International Journal of Molecular Sciences. 2021; 22(4): 1988. https://doi.org/10.3390/ijms22041988
7. Kirpatovskiy V.I., Mudraya I.S., Grekov E.A., Kabanova I.V., Golovanov S.A., Drozhzheva V.V., Nadtochiy O.N. Vliyanie eksperimental'no vyzvannogo metabolicheskogo sindroma na funkcional'noe sostoyanie mochevogo puzyrya u krys. Eksperimental'naya i klinicheskaya urologiya. 2013; (1): 8-13.
8. Korolev Yu.N., Bobrovnickiy I.P., Geniatulina M. S. Mihaylik L.V., Nikulina L.A., Bobkova A.S., Yakovlev M.Yu. Sochetannoe deystvie pit'evoy mineral'noy vody i nizkointensivnogo elektromagnitnogo izlucheniya v usloviyah immobilizacionnogo stressa (eksperimental'noe issle- dovanie). Voprosy kurortologii, fizioterapii i lechebnoy fizicheskoy kul'tury. 2015; 92(6): 37-41. https://doi.org/10.17116/kurort2015637-41
9. Korolev Yu.N., Nikulina L.A., Mihaylik L.V. Profilaktika postradiacionnyh narusheniy v semennikah krys pri primenenii magnitnogo polya. Vestnik vosstanovitel'noy mediciny. 2021; 20 (2): 104-108. https://doi.org/10.38025/2078-1962-2021-20-2-104-108
10. Korolev Yu.N., Mihaylik L.V., Nikulina L.A. Vliyanie nizkointensivnogo elektromagnitnogo izlucheniya na strukturno-metabolicheskie processy u zdorovyh krys. Vestnik vosstanovitel'noy mediciny. 2019; 94 (6): 60-62.
11. Makarova M.N., Makarov V.G. Diet-inducirovannye modeli metabolicheskih narusheniy. Eksperimental'nyy metabolicheskiy sindrom. Laboratornye zhivotnye dlya nauchnyh issledovaniy. 2018; (1): 1-19. https://doi.org/10.29296/2618723X-2018-01-08
12. Pan G., Kong L.D. High fructose diet-induced metabolic syndrome. Pathophysiological mechanism and treatment by traditional Chinese medicine. Pharmacological Research. 2018; (130): 438-450. https://doi.org/10.1016/j.phrs.2018.02.020
13. Leonardi B.F., Gosmann G., Zimmer A.R. Modeling Diet-Induced Metabolic Syndrome in Rodents. Molecular Nutrition Food Reseach. 2020; 64(22): e2000249. https://doi.org/10.1002/mnfr.202000249
14. Moreno-Fernandes S., Garees-Rimon M., Vera G., Astier J., Landrier J.F., Miguel M. High Fat/High Glucose Diet Induces Metabolic Syndrome in an Experimental Rat Model. Nutrients. 2018; 10 (10): 1502. https://doi.org/10.3390/nu10101502
15. El-Mehi A.E., Faried M.A. Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study. Folia Morphologica. 2020; 79(4): 690-708. https://doi.org/10.5603/FM.a2019.0139
16. Aydin S., Aksoy A., Kalayci M., Yilmaz M., Kuloglu T., Citil C., Catac Z. Today's and yesterday's of pathophysiology: Biochemistry of metabolic syndrome and animal models. Nutrition. 2014; 30(1): P.1-9. https://doi.org/10.1016/j.nut.2013.05.013
